Cyto-tox
- Various cell lines from various species and organs, e.g. Vero, L929, HCE-S,… or primary cells (PBMC, BMMC)
Hemolysis
- On red blood cells
We offer biopharmaceutical services tailored to suit your R&D projects as well as face your solubility challenges with your API.
Marinomed Biotech AG offers a wide range of cell based toxicological, immunological or antiviral assays to interrogate the biological activity, toxicity or mechanism of action of a test molecule via our Solv4U services and partnerships program.
Furthermore, we have implemented several in-vitro and ex-vivo decongestion, moisturizing, barrier and permeation assays in order to evaluate potential efficacy of your API or novel formulation. These assays might be your first indication, or comparison to a reference or marketed product, about a potential success of your R&D project at very early stage.
T-cells
- Jurkat cell lines, primary human cells/stimuli PMA-Iono/CD3-CD28 pathways/different cytokines
Dendritic cells
- Cell line DC18C10/stimuli PMA-Iono, LPS/NF-kB pathway/TNF-alpha and other cytokines
Mast cells
- CFTL-12, primary mouse cells/stimuli PMA-Iono, IgE-AG/calcineurin inhibitors (or clobetasol) for TNF-alpha, histamine release, other cytokines and chemokines
Hemagglutination inhibition
- Influenza A viruses endemic and pandemic (e.g., H1N1, H3N2, H7N7)
- Influenza B virus (e.g., Yamagata)
- Human Coronavirus OC43
- Human Parainfluenzavirus 3
Infectivity assay (single round)
- SARS-CoV-2 Lentivirus pseudovirus (Wuhan and variants of concern)
Replication inhibition assay
- Influenza virus A H1N1n, H3N2, H5N1, H7N7
- Human Rhinovirus major and minor group
- Coxsackie A10 + A24
- Herpes simplex virus 1 +2
Neuraminidase inhibition
- Various influenza viruses (RBC or biochemically)
FACS-analysis
- Cell size determination
Cell assays
- Product relevant cells and cell lines, e.g. HCE-S, A549…
Ex-vivo
- Porcine eye – cornea
- Porcine nasal mucosa
In-vitro
- For allergens, particles
In-vitro
- PAMPA (cell-free system)
Ex-vivo
- Porcine eyes (cornea, conjunctiva, aqueous humor, sclera, vitreous, retina,…)
- Porcine nasal mucosa (cavity, septum)
- Porcine stomach (fundus, corpus)
- Porcine oral cavity (lingual, buccal mucosa)
Formulation
- Solubility studies
- Formulation development
- Primary packaging compatibility
Physico-chemical parameters
- Appearance (clarity and color), pH, conductivity
HPLC analytics
- RP-HPLC various detection methods (CAD, DAD, UV/Vis, RI, fluorescence)
- Method development
- Forced degradation studies
Stability studies
- 25°C/60%RH
- 30°C/75%RH
- 40°C/75%RH
Dr. Cornelia Siegl
Head Solv4U